Fig 1: Expression of protein regulator of cytokinesis 1 (PRC1) in tumour and pericarcinomatous tissue from 45 patients with cholangiocarcinoma, assayed in triplicate by western blot. PRC1 protein densitometric values were normalised to GAPDH internal control: (a) representative western blot panels showing PRC1 levels in tumour (N) and pericarcinomatous (P) tissues and comparable GAPDH reference levels among all samples; and (b) relative quantification of PRC1 levels in tumour (N) versus pericarcinomatous (P) tissues (normalised to GAPDH). **P < 0.01 versus pericarcinomatous tissues (Independent samples t-test).
Fig 2: Expression of protein regulator of cytokinesis 1 (PRC1) in tumour and pericarcinomatous tissue from patients with cholangiocarcinoma (CCA), showing: (a) representative photomicrograph of low positive cytoplasmic PRC1 immunosignal in pericarcinomatous tissue (normal control); (b–d) representative photomicrographs of positive cytoplasmic PRC1 immunosignal in tumour tissues; and (e) plot showing overall survival in patients with CCA and high (relative level, 1.3–2.7) or low PRC1 expression (relative level, 0–1.3). Original magnification, × 200.
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